Antioxidant Vitamins and indices of oxidative Stress in sera positive for Rheumatoid Factor

  • Olatunbosun Ganiyu Arinola
Keywords: Rheumatoid factor, auto-antibodies, nutrition, inflammation.


Background: Rheumatoid factor (RF), an autoantibody, is often present in sera of patients suspected of having arthritis, liver diseases, auto-immune disorders, certain viral and bacteria infections and few healthy individuals. Up till now, factors responsible for the RF presence are not fully understood but the involvement of oxidant-antioxidant imbalance has been suggested. Therefore, this study was carried out to determine the serum levels of selected vitamins and oxidative stress indices in RF-positive patients

Methods: Indices of oxidative stress [malondialdehyde (MDA), total peroxide (TP), total antioxidant potential (TAP)] and vitamins C and E were spectrophotometrically determined in 28 patients with RF-positive sera and 28 age-matched apparently healthy adults whose sera were RF-negative (controls). Thereafter, oxidative stress index (OSI) was appropriately calculated. Presence of RF was determined using latex agglutination method. Student’s t-test was used to compare differences in means while Pearson’s correlation analysis was used to establish the correlation between RFtiter and the biochemical indices.

Results: The mean serum levels of TP, MDA and OSI were significantly elevated in RF-positive sera compared with controls. In contrast, the mean serum levels of TAP and vitamin E were significantly lower in RF-positive seracompared with controls.

Conclusion: It could be concluded from this study that increased oxidative stress and reduced antioxidant activity in RF-positive sera may contribute to RF related diseases. Thus, monitored co-administration of antioxidants supplement or increased intake of antioxidant containing diets with conventional drugs might be beneficial inpatients with RF related diseases


Bracht A, Silveira SS, Castro-Ghizoni CV, Sá-Nakanishi AB, Oliveira MR, Bersani-Amado CA, Peralta RM, Comar JF. Oxidative changes in the blood and serum albumin differentiate rats with monoarthritis and polyarthritis. Springerplus2016;5:36.

Chandankhede MS, Gupta MM. Oxidative stress and antioxidant status in patients with rheumatoid arthritis.Int J Biol Med Res 2013; 4(2):3088-3090.

Darlington LG, Stone TW.Antioxidants and fattyacids in the amelioration of rheumatoidarthritis and relateddisorders. Br J Nutr 2001;85(3):251-269.

Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. ClinBiochem 2004; 37:277- 285.

Harma M, Harma M, Erel O. Increased oxidative stress in patients with hydatidiform mole. Swiss Med Wkly 2003; 133:563-566.

Hirao M, Yamasaki N, Oze H, Ebina K, Nampei A, Kawato Y, Shi K, Yoshikawa H, Nishimoto N, Hashimoto J.Serumlevel of oxidativestressmarker is dramaticallylow in patients with rheumatoidarthritistreated with tocilizumab. RheumatolInt 2012;32(12):4041-4045.

Hitchon CA, El-Gabalawy HS. Oxidation in rheumatoid arthritis.Arthritis Res Ther2004;6(6):265-278.

Ingegnoli F, Castelli R, Gualtierotti R. Rheumatoid Factors: Clinical Applications. Disease Markers 2013; 35 (6):727–734.

Işik A, Selek S. Total Antioxidant Response and Oxidative Stress in Patients with Rheumatoid Arthritis. FıratÜniversitesiSağlıkBilimleri Tıp Dergisi 2007; 21(2):067-073.

Jaswal S, Mehta HC, Sood AK, Kaur J. Antioxidantstatus in rheumatoid arthritis and role of antioxidanttherapy.ClinChimActa 2003;338(1-2):123-129.

Kapitány A, Végvári A, Sipka S, Szücs G, Szántó S, Lakos G. Anti-citrullinated protein antibodies in rheumatoid arthritis: as good as it gets?Clin Rev Allergy Immunol 2008; 34(1):26-31.

Karatas F, Ozates I, Canatan H, Halifeoglu I, Karatepe M, Colak R.Antioxidant status & lipid peroxidation in patients withrheumatoid arthritis. Indian J Med Res 2003; 118:178-181.

Khansari N, Shakiba Y and Mahmoudi M. Chronic Inflammation and Oxidative Stress as a Major Cause of Age-Related Diseases and Cancer. Recent Patents on Inflammation & Allergy Drug Discovery 2009; 3:73-80.

Knekt P, Heliövaara M, Aho K, Alfthan G, Marniemi J, Aromaa A. Serumselenium, serumalpha-tocopherol, and the risk ofrheumatoid arthritis.Epidemiology 2000;11(4):402-405.

Kyburz D, Corr M, Brinson DC, Von Damm A, Tighe H, Carson DA. Human rheumatoid factor production is dependent on CD40 signaling and autoantigen. J Immunol1999;163(6):3116-3122.

Mageed RA, Børretzen M, Moyes SP, Thompson KM, Natvig JB. Rheumatoid factor autoantibodies in health and disease. Ann N Y AcadSci1997;815:296-311.

Mateen S, Moin S, Khan AQ, Zafar A, Fatima N. IncreasedReactive Oxygen SpeciesFormation and Oxidative Stress in Rheumatoid Arthritis. PLoS One 2016;11(4):e0152925.

Pashkow FJ. Oxidative Stress and Inflammation in Heart Disease: Do Antioxidants Have a Role in Treatment and/or Prevention? Int J Inflam2011;2011:514623.

Rheumatoid Arthritis and Complementary Health Approaches. National Center for Complementary and Alternative Medicine. D441, Created October 2009, Updated July 2013.Available on: 11th April, 2016.

Satoh K. Serum lipid peroxide in cerebrovascular disorder determined by new colorimetric method. Clin.Chim. Acta 1978; 90: 37-43.

Schubert AC, Wendt MM, de Sá-Nakanishi AB, Amado CA, Peralta RM, Comar JF, Bracht A. Oxidative state and oxidative metabolism of the heart from rats with adjuvant-induced arthritis.ExpMolPathol2016;100(3):393-401.

Song YW, Kang EH. Autoantibodies in rheumatoid arthritis: rheumatoid factors and anticitrullinated protein antibodies. QJM 2010;103(3):139–146.

Traber MG. Vitamin E regulatory mechanisms.Annu Rev Nutr2007;27:347-362.

Wagner BA, Buettner GR, Burns CP. Vitamin E slows the rate of free radical-mediated lipid peroxidation in cells.Arch BiochemBiophys1996;334(2):261-267.

Winrow VR, Winyard PG, Morris CJ, Blake DR.Free radicals in inflammation: second messengers and mediators of tissue destruction. Br Med Bull1993;49(3):506-522.

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